Several software programs are used in the making of PeptideAtlas. The main packages follow:
► LC-MS/MS spectra identification: COMET (UW), MSFragger (U. Michigan), SpectraST (ISB), and others.
► Identification correctness scoring (ISB): Trans-Proteomic Pipeline, including PeptideProphet, iProphet, PTMProphet, and ProteinProphet.
► Database loading and query (ISB): SBEAMS
► Genome Annotation (EMBL-EBI and Sanger Institute): Ensembl
► Peptide mapping to Ensembl (NCBI): BioEnsembl and BioPerl
► Open-source tools for mass spectrometry (ISB): Sashimi, TPP